Journal: Microbial Cell Factories

Article Title: Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

doi: 10.1186/s12934-016-0612-6

Figure Lengend Snippet: Primers, plasmids, and Escherichia coli strains used in this study

Article Snippet: The deleted genes and their corresponding enzymes are as follows: ldhA lactate dehydrogenase; dld d -lactate dehydrogenase; pps phosphoenolpyruvate synthetase; poxB pyruvate oxidase; adhE aldehyde-alcohol dehydrogenase; pta phosphate acetyltransferase; ackA acetate kinase; atoD acetoacetyl-CoA transferase; atoA acetoacetyl-CoA transferase. b Analysis of extracellular metabolites (acetate, lactate, pyruvate, and mevalonate) accumulated during the culture of strain MGpsPtM (wild-type MG1655 harboring pTS-sPtispS-MVA) shown in Fig. ( open squares ) and the strain ApsPtM (the knockout mutant AceCo harboring the same plasmid) shown in Fig. ( closed squares ) after induction with 0.01 mM IPTG Fig. 6 Effects of deletion of genes involved in byproduct formation on isoprene production.

Techniques: Expressing, Plasmid Preparation

Journal: Microbial Cell Factories

Article Title: Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

doi: 10.1186/s12934-016-0612-6

Figure Lengend Snippet: Effects of low IPTG concentrations on isoprene production and cell growth of the MGpsPtM strain (MG1655 harboring pTS-sPtispS-MVA). Culture was carried out in TB medium containing 2.0% (w/v) glycerol for 36 h at 30 °C. IPTG was initially added at concentrations of 0 mM ( open squares ), 0.01 mM ( closed squares ), 0.02 mM ( closed triangles ), and 0.03 mM IPTG ( closed circles )

Article Snippet: The deleted genes and their corresponding enzymes are as follows: ldhA lactate dehydrogenase; dld d -lactate dehydrogenase; pps phosphoenolpyruvate synthetase; poxB pyruvate oxidase; adhE aldehyde-alcohol dehydrogenase; pta phosphate acetyltransferase; ackA acetate kinase; atoD acetoacetyl-CoA transferase; atoA acetoacetyl-CoA transferase. b Analysis of extracellular metabolites (acetate, lactate, pyruvate, and mevalonate) accumulated during the culture of strain MGpsPtM (wild-type MG1655 harboring pTS-sPtispS-MVA) shown in Fig. ( open squares ) and the strain ApsPtM (the knockout mutant AceCo harboring the same plasmid) shown in Fig. ( closed squares ) after induction with 0.01 mM IPTG Fig. 6 Effects of deletion of genes involved in byproduct formation on isoprene production.

Techniques:

Journal: Microbial Cell Factories

Article Title: Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

doi: 10.1186/s12934-016-0612-6

Figure Lengend Snippet: a Pathway of byproduct formation from acetyl-CoA and pyruvate in Escherichia coli . Strikeouts indicate deleted genes in the AceCo strain. The deleted genes and their corresponding enzymes are as follows: ldhA lactate dehydrogenase; dld d -lactate dehydrogenase; pps phosphoenolpyruvate synthetase; poxB pyruvate oxidase; adhE aldehyde-alcohol dehydrogenase; pta phosphate acetyltransferase; ackA acetate kinase; atoD acetoacetyl-CoA transferase; atoA acetoacetyl-CoA transferase. b Analysis of extracellular metabolites (acetate, lactate, pyruvate, and mevalonate) accumulated during the culture of strain MGpsPtM (wild-type MG1655 harboring pTS-sPtispS-MVA) shown in Fig. ( open squares ) and the strain ApsPtM (the knockout mutant AceCo harboring the same plasmid) shown in Fig. ( closed squares ) after induction with 0.01 mM IPTG

Article Snippet: The deleted genes and their corresponding enzymes are as follows: ldhA lactate dehydrogenase; dld d -lactate dehydrogenase; pps phosphoenolpyruvate synthetase; poxB pyruvate oxidase; adhE aldehyde-alcohol dehydrogenase; pta phosphate acetyltransferase; ackA acetate kinase; atoD acetoacetyl-CoA transferase; atoA acetoacetyl-CoA transferase. b Analysis of extracellular metabolites (acetate, lactate, pyruvate, and mevalonate) accumulated during the culture of strain MGpsPtM (wild-type MG1655 harboring pTS-sPtispS-MVA) shown in Fig. ( open squares ) and the strain ApsPtM (the knockout mutant AceCo harboring the same plasmid) shown in Fig. ( closed squares ) after induction with 0.01 mM IPTG Fig. 6 Effects of deletion of genes involved in byproduct formation on isoprene production.

Techniques: Knock-Out, Mutagenesis, Plasmid Preparation

Journal: Microbial Cell Factories

Article Title: Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts

doi: 10.1186/s12934-016-0612-6

Figure Lengend Snippet: Stepwise increases in isoprene production from the engineered Escherichia coli strains

Article Snippet: The deleted genes and their corresponding enzymes are as follows: ldhA lactate dehydrogenase; dld d -lactate dehydrogenase; pps phosphoenolpyruvate synthetase; poxB pyruvate oxidase; adhE aldehyde-alcohol dehydrogenase; pta phosphate acetyltransferase; ackA acetate kinase; atoD acetoacetyl-CoA transferase; atoA acetoacetyl-CoA transferase. b Analysis of extracellular metabolites (acetate, lactate, pyruvate, and mevalonate) accumulated during the culture of strain MGpsPtM (wild-type MG1655 harboring pTS-sPtispS-MVA) shown in Fig. ( open squares ) and the strain ApsPtM (the knockout mutant AceCo harboring the same plasmid) shown in Fig. ( closed squares ) after induction with 0.01 mM IPTG Fig. 6 Effects of deletion of genes involved in byproduct formation on isoprene production.

Techniques: